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Recent urban public water supply contamination events emphasize the importance of screening treated drinking water quality after distribution. In vitro bioassays, when run concurrently with analytical chemistry methods, are effective tools to evaluating the efficacy of water treatment processes and water quality. We tested 49 water samples representing the Chicago Department of Water Management service areas for estrogen, (anti)androgen, glucocorticoid receptor-activating contaminants and cytotoxicity. We present a tiered screening approach suitable to samples with anticipated low-level activity and initially tested all extracts for statistically identifiable endocrine activity; performing a secondary dilution-response analysis to determine sample EC50 and biological equivalency values (BioEq). Estrogenic activity was detected in untreated Lake Michigan intake water samples using mammalian (5/49; median 0.21 ng E2Eq/L) and yeast cell (5/49; 1.78 ng E2Eq/L) bioassays. A highly sensitive (anti)androgenic activiistical stringency, and detected estrogenic and androgenic activity only in pre-distribution Chicago area samples.Copper ferrite/reduced graphene oxide (CF/rGO) nanocomposites (NCs) was synthesized using the bio-combustion method and applied as a cathode catalyst in the microbial reduction of CO2 to volatile fatty acids (VFAs) in a single chamber microbial electrosynthesis system (MES). The synthesized NCs exhibited a porous network-like structure with a high surface area of CF/rGO (158.22 m2/g), which was 2.24 folds higher than that of CF. The Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) analysis for CF/rGO/Carbon cloth (Cc) revealed a high reduction current density of -7.3 A/m2 and a low charge transfer resistance of 2.8 Ω. The isobutyrate and acetate in MES-2 (Cu/rGO/Cc) were produced at 35.37 g/m2/d, which was 1.53 folds higher than that of MES-1 (bare Cc 23.10 g/m2/d). The columbic efficiency (77.78%) and total VFA concentration (1941.13 ± 83 mg COD/L) were noted to be 1.97 and 1.6 folds higher for MES-2 than MES-1, respectively. The Tafel plot drawn from the CV curves exhibited an exchange current density value of MES-2 that was 3.46 A/m2, and this value was 1.19 and 33.92 folds higher than that of MES-1 and abiotic CF/rGO/Cc, respectively. Field emission scanning electron microscopy (FESEM) observations revealed enhanced rod-shaped bacteria had grown on the cathode suggesting excellent biocompatible and multi-length scale porosity of CF/rGO catalysts for enhanced colonization of microbes. The phyla Proteobacteria (Betaproteobacteria), Bacteroidetes, and Firmicutes were highly abundant as the dominant microbial communities on the cathode, which might played a major role in bioelectrochemical CO2 reduction to VFAs. The results from this study clearly demonstrate that the CF/rGO/Cc electrode could serve as a conductive element between microbes and bactericidal electrodes with excellent electrochemical properties to enable performance of the MES.Rivers are important for drinking water supply worldwide. However, they are often impacted by pathogen discharges via wastewater treatment plants (WWTP) and combined sewer overflows (CSO). To date, accurate predictions of the effects of future changes and pollution control measures on the microbiological water quality of rivers considering safe drinking water production are hindered due to the uncertainty of the pathogen source and transport variables. The aim of this study was to test an integrative approach for an improved understanding of these effects, i.e. climate change and population growth as well as enhanced treatment at WWTPs and/or prevention of CSOs. We applied a significantly extended version of QMRAcatch (v1.0 Python), a probabilistic-deterministic model that combines fate and transport modelling with quantitative microbial infection risk assessment. The impact of climatic changes until the period 2035-2049 was investigated by a conceptual semi-distributed hydrological model, based on regional cis applicable at other catchments and pollution sources. It allows assessing future changes and selecting suitable pollution control measures for long-term water safety planning.Elderly residents are prominent users of urban parks and comfortable open spaces in parks have been shown to improve their physical health and mental well-being. In this study, the thermal perceptions (thermal sensation, thermal comfort and thermal acceptability) of elderly visitors to an urban park in Xi'an, China was investigated using meteorological measurements, questionnaire surveys and activity records. Physiological equivalent temperature (PET) was used to determine thermal benchmarks. Spatial-temporal distributions of the elderly in open spaces were recorded and relationships among elderly residents' thermal perceptions, their age and chronic disease were analyzed. Finally, optimal design strategies for open spaces suitable for the elderly were proposed based on meteorological characteristics, elderly residents' attendance patterns and their thermal perceptions. [https://www.selleckchem.com/products/azd0156-azd-0156.html selleck compound] Results showed that 1) globe temperature (Tg) and air temperature (Ta) were the primary meteorological factors that influenced elderly residelogical preferences.The widespread use of silver nanoparticles (Ag NPs) has raised substantial health risks, but little is known about the epigenetic toxicity induced by Ag+ and Ag NPs. This study characterized physiological and lncRNA profiles to explore the toxic effects and epigenetic mechanisms in Tetrahymena thermophila on exposure to Ag+ (in the form of AgNO3) and different Ag NPs for 24 h. The Ag NPs studied varied in size (10 nm and 80 nm) and surface coating (citrate and polyvinylpyrrolidone). We found that both Ag+ and Ag NPs elicited strong growth-inhibiting effects on T. thermophila. The toxicity was mainly caused by high reactive oxygen species (ROS) levels, leading to lipid peroxidation and mitochondrial dysfunction. To combat the oxidative stress, the protist activated an antioxidative response, increasing the activity of glutathione peroxidase and other antioxidants. Notably, 1250 lncRNAs were differentially expressed under Ag+ or Ag NPs exposure relative to the non-exposure control, which were clustered into 15 expression modules in weighted gene co-expression network analysis.