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In the control group, ST1 (21.8%), ST2 (21.8%), ST3 (43.5%), ST6 (4.4%) and ST8 (8.7%) were identified. This study is the first report regarding the occurrence and subtypes distribution of Blastocystis in patients with diabetes mellitus in Brazil. The results reinforce the potential risk of Blastocystis infection in patients with diabetes, in addition, it contributes to the understanding of the genetic diversity of this enigmatic organism.Chagas cardiomyopathy (ChCM) is a severe consequence of Trypanosoma cruzi infection and has a range of electrocardiographic (ECG) and echocardiographic (ECHO) manifestations. There is a need for a standard and parsimonious research cardiac end point that does not rely on expert panel adjudication, and it is not intended to change the ChCM definition. We use data from the REDS-II cohort to propose a simplified cardiac endpoint. A total of 499 T. cruzi-seropositive blood donors were included. All participants underwent a 12-lead ECG, echocardiogram and clinical examination, and those with abnormal findings were reviewed by a panel of cardiologists who classified cases as having Chagas cardiomyopathy or not. We created an exhaustive set of ECG and ECHO finding combinations and compared these with the panel's classification. We selected the simplest combination that most accurately reproduced the panel's results. Individual ECG and ECHO variables had low sensitivity for panel-defined cardiomyopathy. The best performing combination was right bundle branch block and/or ECHO evidence of left ventricular hypocontractility. [https://www.selleckchem.com/products/gsk2606414.html Selleck GSK2606414] This combination had 98% specificity and 85% sensitivity for panel-defined ChCM. It was not possible to improve the overall accuracy by addition of any other ECG or ECHO variable. Substituting right bundle branch block for the more inclusive finding of QRS interval &gt; 120 ms produced similar results. The combination of prolonged QRS interval and/or left ventricular hypocontractility closely reproduced the REDS-II expert panel classification of Chagas ChCM. In conclusion, the simple and reproducible research endpoint proposed here captures most of the spectrum of cardiac abnormalities in Chagas disease.<br />To verify the validity evidence based on response processes of a vocabulary-screening tool.<br />This is a descriptive, cross-sectional and quantitative study, applied in a sample of 133 children between 3 and 7 years of age, divided into five groups, according to their age range. This research evaluates the instrument TRILHAR, which is a screening of receptive and expressive vocabulary, composed by ten activities for each age range. The collected data were the application time in minutes and seconds, screening instructions comprehension and behavior during the screening. The data were analyzed descriptively as mean, standard deviation and percentage using the IBM SPSS Statistics.<br />The application time was short, with less time for the greater ages. The mean time of application was 05m19s for G1, 04m29s for G2, 04m11s for G3, 03m40s for G4 and 02m13s for G5. Only one child (4.8%) from G2 required repetition of the instructions for the receptive vocabulary, and two children (6.3%) from G4 for the expressive vocabulary. We observed little occurrence of behaviors like disinterest, inattention and agitation.<br />The application of the instrument required a short period, with a longer time for the group of 3-years children. The little necessity of repetition of the instructions indicates that children can easily comprehend the instrument.<br />The application of the instrument required a short period, with a longer time for the group of 3-years children. The little necessity of repetition of the instructions indicates that children can easily comprehend the instrument.<br />To verify the immediate effect of the Excitomotor Electrical Current, called Functional Electrical Stimulation (FES), on vocal quality, Maximum Phonation Time (MPT) and possible discomfort, in women without vocal alteration, with application at Maximum Supported Intensity (MSI) and associated with phonation.<br />Experimental study with 20 normophonic adult women. They emitted the sustained vowel / a / and then it was applied to FES during emission of the same vowel. There were five series with three minutes of emission each, interspersed with passive rest. The electrical stimulus was at the MSI by the participant, adjusted by series. Before and after the emissions the voices were recorded and the MPT and the intensity of the stimuli were collected. The vocal quality was rated by judges. Statistical analysis made it possible to compare pre and post emission / electrostimulation data in each phase. Qualitative analysis was performed based on self-reported symptoms.<br />There was no difference in vocal quality and MPT between pre and post moments in both phases. The difference between MSI and stimulus perception intensity was greater in series 1 than in series 2. There was an increase in MSI in series 5 compared to series 1. No significant negative symptoms or within 48h after procedures were reported.<br />The FES at MSI, associated with phonation, did not generate an immediate change in vocal quality, in the MPT or self-reported discomforts by women without vocal alteration, even with a gradual increase in the stimulus, series by series.<br />The FES at MSI, associated with phonation, did not generate an immediate change in vocal quality, in the MPT or self-reported discomforts by women without vocal alteration, even with a gradual increase in the stimulus, series by series.<br />To propose the Dysphonia Risk Screening Protocol for Musical Theatre Actors (DRSP-MTA), to verify its applicability in association with the General Dysphonia Risk Screening Protocol (G-DRSP), to correlate the final scores of both, and these with the total risk score, and to compare the risk of dysphonia measured in musical theater actors with and without vocal complaint.<br />An observational cross-sectional study with 34 musical theater actors, adults, of both genders, with and without vocal complaints and regardless of whether they are professionals or students. The questionnaires were applied individually. Statistical analysis made it possible to verify the correlation between the dysphonia risk scores and to compare the groups with and without vocal complaint.<br />Most of the participants were male, young adults, professional actors and without vocal complaint. There was a high risk of dysphonia, evidenced by the application of G-DRSP, with means scores compatible with values found in individuals with dysphofferentiation.<br />To evaluate how the auditory pathways encode and discriminate the plosive syllables [ga], [da] and [ba] using the auditory evoked Frequency-following Response (FFR) in children with typical development.<br />Twenty children aged 6-12 years were evaluated using the FFR for the [ga], [da] and [ba] stimuli. The stimuli were composed of six formants and were differentiated in the F2 to F3 transition (transient portion). The other formants were identical in the three syllables (sustained portion). The latencies of the 16 waves of the transient portion (&lt;70ms) and of the 21 waves of the sustained portion (90-160ms) of the stimuli were analyzed in the neural responses obtained for each of the syllables.<br />The transient portion latencies were different in the three syllables, indicating a distinction in the acoustic characteristics of these syllables through their neural representations. In addition, the transient portion latencies progressively increased in the following order [ga] &lt;[da] &lt;[ba], whereas no significant differences were observed in the sustained portion.<br />The FFR proved to be an efficient tool to investigate the subcortical acoustic differences in speech sounds, since it demonstrated different electrophysiological responses for the three evoked syllables. Changes in latency were observed in the transient portion (consonants) but not in the sustained portion (vowels) for the three stimuli. These results indicate the neural ability to distinguish between acoustic characteristics of the [ga], [da] and [ba] stimuli.<br />The FFR proved to be an efficient tool to investigate the subcortical acoustic differences in speech sounds, since it demonstrated different electrophysiological responses for the three evoked syllables. Changes in latency were observed in the transient portion (consonants) but not in the sustained portion (vowels) for the three stimuli. These results indicate the neural ability to distinguish between acoustic characteristics of the [ga], [da] and [ba] stimuli.This study evaluated the use of gill parasitic monogenoideans from Sciades herzbergii (Siluriformes Ariidae) as bioindicators of environmental quality in Amazonian estuarine ecosystems. Fish were caught in the tidal channels in an impacted area - IA, near the port of São Luís and a reference area - RA, in the Caeté estuary, Bragança. link2 The influence of the capture site and seasonality on parasite abundance, environmental variables and biometric data of hosts were verified. A total of 416 S. herzbergii specimens were analyzed. link3 The weight and total length of fish from the RA were greater than those of the IA. The relative condition factor was influenced by seasonality. A total of 4,265 monogenoidean specimens from the following species were identified Chauhanellus boegeri, C. susamlimae, C. velum, Calceostomella herzbergii and Dactylogyridae gen. sp. The seasonal mean parasitic abundance of C. susamlimae was significantly different, with the highest values recorded during the dry season. The mean abundance of C. herzbergii was influenced by seasonality and by the capture site. During the rainy period, the parasite indices in the RA were higher than those of IA. C. herzbergii is a bioindicator sensitive to low water quality, especially in relation to water turbidity in Amazon estuaries.Fish consumption plays an important role in human diet. Hoplias malabaricus, commonly known as traíra, is a freshwater fish widely appreciated in several Brazilian states and frequently infected by Eustrongylides sp. fourth-instar larvae (L4). The aim of the present study was to evaluate allergenic potential of Eustrongylides sp. L4 crude extract (CEE). BALB/c mice were immunized intraperitoneally (IP) by 10 μg CEE with 2 mg of aluminum hydroxide on days 0 and 35. Specific IgG and IgE antibody levels were determined after immunization and cellular immunity was evaluated by assessing intradermal reaction in ear pavilion. Epicutaneous sensitization was performed in dorsal region by antigen exposure using a Finn-type chamber containing 50 μg of CEE or saline solution, followed by evaluation of specific antibody levels. IP immunization resulted in a gradual increase in IgG antibody levels and transitory IgE production. Significant increase in ear thickness was observed in cellular hypersensitivity reaction. In case of antigen exposure by epicutaneous route, CEE was able to induce meaningfully increased levels of specific IgG and IgE antibodies as well as heightened cellular immunity. Both intraperitoneal immunization and epicutaneous contact with Eustrongylides sp. larval antigens were observed for first time to be capable of inducing immunological sensitization in mice.