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g., feeding suppression) to oil pollution in zooplankton.
Congenital fibrinogen disorders result from genetic mutations in FGA, FGB, or FGG resulting in quantitative fibrinogen deficiencies (afibrinogenemia or hypofibrinogenemia) or qualitative fibrinogen deficiencies (dysfibrinogenemia). Hypodysfibrinogenemia sharing features with hypo- and dysfibrinogenemia is rare. We performed genetic and functional analyses of a 31-year-old woman with suspected hypodysfibrinogenemia.
Functional and antigenic fibrinogen values of patient were 1.05 and 1.24 g/L, respectively. DNA sequence and western blotting analyses for plasma fibrinogen were performed. A minigene incorporating the mutational region was transfected into a Chinese hamster ovary cell line (CHO), and reverse transcription products were analyzed. Assembly and secretion were examined using the recombinant variant fibrinogen. We purified the patient's plasma fibrinogen and analyzed thrombin-catalyzed fibrin polymerization (TCFP).
DNA sequencing revealed compound heterozygous nucleotide mutations with FGB 35 bp nalysis. A minigene incorporating the deletion DNA showed two aberrant mRNA products. The secretion of Bβp.N170K-fibrinogen-CHO was almost same as normal Bβ-fibrinogen-CHO. TCFP of plasma Bβp.N170K fibrinogen was slightly lower than that of normal plasma fibrinogen. Aberrant splicing products derived from the 35 bp deletion caused hypofibrinogenemia due to nonsense-mediated mRNA decay and suggested the presence of only Bβp.N170K fibrinogen in patient's plasma. Bβp.N170K caused dysfibrinogenemia due to a delay in lateral aggregation. These findings demonstrated that these mutations respectively affected the fibrinogen quality and quantity, resulting in hypodysfibrinogenemia.A Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) has become a pandemic disease named Coronavirus Disease-19 (COVID-19) of epochal dimension. The clinical spectrum of COVID-19 is wide, ranging from asymptomatic forms to severe pneumonia, sepsis and multiple organ dysfunction syndromes resulting in poor outcomes. Among the various consequences of severe COVID-19, cardiovascular (CV) collapse appears the most serious and potentially lethal. On the other hand, pre-existent CV comorbidities are also associated with higher mortality. The most reliable hypothetical pathogenetic mechanism for CV complications and cardiac injury in severe COVID-19 patients appears to be a sustained endothelial dysfunction, caused by the interplay of inflammation and coagulation. In this review, we survey papers addressing issues related to severe COVID-19, characterized by enhanced lung microvascular loss, hypercytokinemia, hypoxemia and thrombosis. We discuss about how the virus-induced downregulation of the angiotensin converting enzyme-2 (ACE2) receptor, used to enter the host cell, could affect the renin-angiotensin system, attempting to clarify the doubts about the use of ACE inhibitors and Angiotensin-II receptor blockers in COVID-19 patients. Finally, we point out how the delicate and physiological homeostatic function of the endothelium, which turns into a disastrous battlefield of the complex interaction between "cytokine and coagulative storms", can be irreparably compromised and result in systemic inflammatory complications.
The effects of the environmental factors on successful aging (SA) are not well understood. This study aimed to assess SA and related factors in older individuals in urban and rural areas, exploring differences between groups and investigating the effects of environmental factors.
This was a cross-sectional study of 205 and 212 older people in urban and rural areas of Shandong Province, respectively, between March 2019 and September 2019. SA was measured using the Successful Aging Inventory (SAI). The environmental factors were assessed using the WHOQOL-100 scale. Univariable and multivariable analyses were performed to determine associations of different parameters with SA.
The scores of SA and environmental factors of older individuals in urban vs. buy Sodium L-lactate rural areas were 48.79 vs. 46.14 and 128.63 vs. 107.81, respectively (both P < 0.05). All "Environment" dimensions ("Safety and physical security", "Home environment", "Financial resources", "Health and social care", "Opportunities for acquiring new information and skills", "Participation and opportunities for leisure", and "Transport"), except "Physical environment (pollution/noise/traffic/climate)", were associated with SA (all P < 0.05). Multiple linear regression showed that psychological resilience, physical activity, self-evaluation of SA, environment, social support, and hearing status were shared factors by the urban and rural older individuals.
The SA and environmental factor scores were higher in urban older individuals compared with rural ones. Environment dimensions (except "Physical environment (pollution/noise/traffic/climate)") were associated with SA.
The SA and environmental factor scores were higher in urban older individuals compared with rural ones. Environment dimensions (except "Physical environment (pollution/noise/traffic/climate)") were associated with SA.In this study, lipid oxidation evaluation methods were compared for a krill-oil-in-water emulsion system. With this aim, thiocyanate and DPPP (diphenyl-1-pyrenylphosphine) fluorescence methods were comparatively examined to determine primary oxidation products. 2-thiobarbituric acid reactive substances (TBARS), hexanal and propanal formation were also monitored as secondary oxidations products. All oxidation experiments were performed via both auto-oxidation at 45 °C and light-riboflavin induced photooxidation at 37 °C. The results have shown that thiocyanate method was not suitable to measure lipid hydroperoxides by the both in auto- and photo-oxidation systems. On the other hand, fluorescence intensity of samples containing the DPPP probe increased during incubation period which indicates the formation of lipid hydroperoxides could be detected via this method. TBARS, hexanal and propanal concentrations also increased during storage period and the formation kinetics of secondary oxidation products was confirmed that the DPPP fluorescence method was accurate and reliable at different environmental conditions.