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The QIAstat-Dx® Respiratory Panel V2 (RP) is a novel molecular-based syndromic test for the simultaneous and rapid (∼70 minutes) detection of 18 viral and three bacterial pathogens causing respiratory infections. This study describes the first multicenter retrospective comparison of the performance of the QIAstat-Dx® RP assay to the established ePlex® Respiratory Pathogen Panel (RPP) assay, for which we used 287 respiratory samples from patients suspected with respiratory infections. The QIAstat-Dx® RP assay detected 312 of the 338 respiratory targets (92%) that were detected by the ePlex® RPP assay. Most discrepant results have been observed in the low pathogen load samples. In addition, the QIAstat-Dx® RP assay detected 19 additional targets in 19 respiratory samples that were not detect by the ePlex® RPP assay. Nine of these discordant targets were considered to be true positives after discrepancy testing by a third method. The main advantage of the QIAstat-Dx® system compared to other syndromic testing systems, including the ePlex® RPP assay, is the ability to generate CT values that could help with the interpretation of results. Taken together, this study shows a good performance of the QIAstat-Dx® RP assay in comparison to the ePlex® RPP assay for the detection of respiratory pathogens. The QIAstat-Dx® RP assay offers a new, rapid, and accurate sample-to-answer multiplex panel for the detection of the most common viral and bacterial respiratory pathogens and therefore has the potential to direct appropriate therapy and infection control precautions. Copyright © 2020 Boers et al.Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and faster turnaround time. The Panther Fusion® (Fusion, Hologic) has an array of highly sensitive, in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses including FluA/B/RSV (FFABR). Fusion has Open Access functionality to perform LDTs alongside IVDs. We developed two LDTs for FluA strain typing on the Panther Fusion, enabling side by side testing with FFABR. Solcitinib chemical structure LDT-FAST uses proprietary primers and probes designed by Hologic for Prodesse Pro-FAST+ (PFAST). exWHO-FAST is an expanded redesign of the WHO recommended RT-PCRs. To evaluate their performance, 110 FluA positive samples were tested. Of these, 104 previously subtyped, 54 were H3, 46 were 09H1, and 4 were fsH1. All were appropriately subtyped by both LDTs. Of the FluA, untyped samples, 3 were subtyped as H3 by both LDTs and 2 were subtyped H3 by LDT-FAST only. The sample not subtyped by either LDT was retested with FFABR and was now negative. Limit of detection (LOD) analyses were performed with five FluA strains. The LDT-FAST LODs were similar to FFABR, while the exWHO-FAST LODs were higher with two H3N2 strains, findings which were explained by analysis of primer/probe homology . In conclusion, either FluA typing assay would be a valuable complement to the Panther Fusion respiratory menu given the performance of these LDTs, the system's full automation, and the ability to split eluates for both IVD and LDT testing. Copyright © 2020 Stellrecht et al.Mycoplasma bovis is a leading cause of pneumonia in modern calf rearing. Fast identification is essential to ensure appropriate antimicrobial therapy. Therefore, the objective of this study was to develop a protocol to identify M. bovis from bronchoalveolar lavage fluid (BALf) with MALDI-TOF MS and to determine diagnostic accuracy in comparison with other techniques. BALf was obtained from 104 cattle and presence of M. bovis was determined in three ways (1) BALf was enriched and after 24, 48 and 72 hours of incubation analyzed by MALDI-TOF MS (RIMM), (2) triplex real-time PCR for M. bovis, M. bovirhinis and M. dispar and (3) ten day incubation on a selective-indicative agar. Diagnostic accuracy of the three tests was determined with Bayesian latent class modelling (BLCM). After 24h of enrichment, M. bovis was identified by MALDI-TOF MS in 3 out of 104 BALf samples. After, 48 and 72h of enrichment, 32/104 and 38/100 samples were M. bovis positive, respectively. Lipase positive Mycoplasma-like colonies were seen in 28 of 104 samples. Real-time PCR resulted in 28/104 positive and 12/104 doubtful results for M. bovis The BLCM showed a sensitivity (Se) and specificity (Sp) of 86.6% (Confidence Interval 69.4%-97.6%) and 86.4% (CI 76.1-93.8) for RIMM. For real-time PCR Se was 94.8% (89.9-97.9) and Sp 88.9% (78.0-97.4). For selective-indicative agar, Se and Sp were 70.5% (52.1-87.1) and 93.9% (85.9-98.4), respectively. These results implicate that rapid identification of M. bovis with MALDI-TOF MS after an enrichment procedure is a promising test for routine diagnostics in veterinary laboratories. Copyright © 2020 American Society for Microbiology.OBJECTIVE In 2017, the American Academy of Pediatrics introduced a new guideline (2017 Clinical Practice Guideline of the American Academy of Pediatrics [AAP 2017]) to diagnose arterial hypertension (HTN) in children that included revised, lower normative blood pressure (BP) values and cut points for diagnosing high BP in adolescents. We studied the impact of the new AAP 2017 guideline on prevalence of HTN in children with type 1 diabetes mellitus (T1DM). RESEARCH DESIGN AND METHODS Up to September 2018, 1.4 million office BP measurements in 79,849 children and adolescents (aged 5-20 years) with T1DM have been documented in the DPV (Diabetes Prospective Follow-up) registry. BP values of the most recent year were aggregated, and BP values of 74,677 patients without antihypertensive medication were analyzed (median age 16 years and diabetes duration 5.3 years and 52.8% boys). BP values were classified according to AAP 2017 and the references of the German Health Interview and Examination Survey for Children andP in children, particularly in patients with increased risk for cardiovascular disease. © 2020 by the American Diabetes Association.OBJECTIVE The association between high glycemic variability and all-cause mortality has been widely investigated in epidemiological studies but rarely validated in glucose-lowering clinical trials. We aimed to identify the prognostic significance of visit-to-visit HbA1c variability in treated patients in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) trial population. RESEARCH DESIGN AND METHODS We studied the risk of all-cause mortality in relation to long-term visit-to-visit HbA1c variability, expressed as coefficient of variation (CV), variability independent of the mean (VIM), and average real variability (ARV), from the 8th month to the transition. Multivariable Cox proportional hazards models were used to estimate adjusted hazard ratio (HR) and 95% CI. RESULTS Compared with the standard therapy group (n = 4,728), the intensive therapy group (n = 4,755) had significantly lower mean HbA1c (6.6% [49 mmol/mol] vs. 7.7% [61 mmol/mol], P less then 0.0001) and lower CV, VIM, and ARV (P less then 0.