VISPRonline a new webbased interactive tool to visualize CRISPR screening process studies

Conclusion. Due to the low participation rate among invited companies, this study is not able to draw any conclusions on whether the digital tool can be used to increase the number of risk assessments.We explore the mechanism of electron transfers mediated by cytochrome c, a soluble protein involved in mitochondrial oxidative phosphorylation and cytochrome b5, a microsomal membrane protein acting as a redox aide in xenobiotic metabolism. We found minimal conservation in the sequence and surface amino acid residues of cytochrome c/b5 proteins among divergent species. Therefore, we question the evolutionary logic for electron transfer (ET) occurring through affinity binding via recognition of specific surface residues/topography. Also, analysis of putative protein-protein interactions in the crystal structures of these proteins and their redox partners did not point to any specific interaction logic. A comparison of the kinetic and thermodynamic constants of wildtype vs. mutants did not provide strong evidence to support the binding-based ET paradigm, but indicated support for diffusible reactive species (DRS)-mediated process. Topographically divergent cytochromes from one species have been substituted for reaction with proteins from other species, implying the involvement of non-specific interactions. We provide a viable alternative (murburn concept) to classical protein-protein binding-based long range ET mechanism. To account for the promiscuity of interactions and solvent-accessible hemes, we propose that the two proteins act as non- specific redox capacitors, mediating one-electron redox equilibriums involving DRS and unbound ions.Communicated by Ramaswamy H. Sarma.We investigated the awareness of occupational hazards, and attitudes and practices towards the use of personal protective equipment (PPE) among informal woodworkers of the Sokoban Wood Village in Ghana. Structured questionnaires were used to collect primary data from 355 woodworkers and analysed using descriptive statistics and logistic regression. The results showed that the workers are aware of PPE and knew about injuries they are exposed to. Most woodworkers had a positive attitude in terms of their perception on the necessity to use PPE but had a low frequency of PPE usage. Educational level, job type, length of service and marital status are the socio-demographic characteristics that significantly influence woodworkers' compliance with health and safety regulations. We recommend that institutions charged with enforcement of occupational health and safety regulations should extend their monitoring roles to informal workers and liaise with local companies to supply PPE to informal woodworkers at subsidized costs.Much of the sustained attention on pandemic preparedness has focused on the ethical justification for plans for the "crisis" phase of a surge when, despite augmentation efforts, the demand for life-saving resources outstrips supply. The ethical frameworks that should guide planning and implementation of the "contingency" phase of a public health emergency are less well described. The contingency phase is when strategies to augment staff, space, and supplies are systematically deployed to forestall critical resource scarcity, reduce disproportionate harm to patients and health care providers, and provide patient care that remains functionally equivalent to conventional practice. We describe an ethical framework to inform planning and implementation for COVID-19 contingency surge responses and apply this framework to 3 use cases. Examining the unique ethical challenges of this mediating phase will facilitate proactive ethics conversations about healthcare operations during the contingency phase and ideally lead to ethically stronger health care practices.Blood hemoglobin (Hb), known to transport oxygen, is the most abundant globular protein in humans. Erythrocytes have ∼10-3 M concentration of ATP in steady-state and we estimate that this high amounts cannot be formed from 10-4 - 10-7 M levels of precursors via substrate-level phosphorylation of glycolysis. check details To account for this discrepancy, we propose that Hb serves as a 'murzyme' (a redox enzyme working along the principles of murburn concept), catalyzing the synthesis of the major amounts of ATP found in erythrocytes. This proposal is along the lines of our earlier works demonstrating DROS (diffusible reactive oxygen species) mediated ATP-synthesis as a thermodynamically and kinetically viable mechanism for physiological oxidative phosphorylation. We support the new hypothesis for Hb with theoretical arguments, experimental findings of reputed peers and in silico explorations. Using in silico methods, we demonstrate that adenosine nucleotide and 2,3-bisphosphoglycerate (2,3-BPG) binding sites are located suitably on the monomer/tetramer, thereby availing facile access to the superoxide emanating from the heme center. Our proposal explains earlier reported in situ experimental findings/suggestions of 2,3-BPG and ADP binding at the same locus on Hb. The binding energy is in the order of 2,3-BPG > NADH > ATP > ADP > AMP and agrees with earlier reports, potentially explaining the bioenergetic physiology of erythrocytes. Also, the newly discovered site for 2,3-BPG shows lower affinity in fetal Hb (as compared to adults) explaining oxygen transfer from mother to embryo. The findings pose significant implications in routine physiology and pathologies like sickle cell anemia and thalassemia.Communicated by Ramaswamy H. Sarma.ABSTRACTHerein, a series of Fe-Ti bimetal oxide adsorbents were prepared by reduction-co-precipitation method, and their performance in removing low concentration H2S at room temperature was investigated. The adsorbents were characterized by X-Ray diffraction (XRD), Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Ultraviolet Visible diffuse reflectance spectroscopy (UV-Vis-DRS), X-Ray photoelectron spectroscopy (XPS) and N2 adsorption-desorption. The results showed that the addition of Ti increased the specific surface area, pore volume and small oligomeric Fe2O3 of ferrihydrite. When the Fe/Ti molar ratio was 81, Fe-Ti bimetal oxide formed a large amount of oligomeric Fe2O3, and its specific surface area and pore volume reached 344.99 m2/g and 0.34 cm3/g, respectively. At this time, Fe-Ti bimetal oxide exhibited the highest breakthrough sulfur capacity of 222.8 mg/g. High temperature calcination caused Fe-Ti bimetal oxide to form small specific surface area and pore volume, and produced crystalline α-Fe2O3.